Publications
Books (Journal Publications Below)
Farmpedia: The Encyclopedia for Small Scale Farmers (2022) (Farmpedia.org) (MN Raizada, Editor; J Wilker, Associate Editor)
This ebook consists of 175 chapters that critically analyze appropriate agro-ecological technologies for subsistence farmers (including beneficial microbial inoculants) with practical tips to help get started, with language that is easy to understand. It is intended for NGOs, charitable groups, agricultural extension officers, farmers and scientists. It is open access, free and available online at www.Farmpedia.org
This ebook consists of 175 chapters that critically analyze appropriate agro-ecological technologies for subsistence farmers (including beneficial microbial inoculants) with practical tips to help get started, with language that is easy to understand. It is intended for NGOs, charitable groups, agricultural extension officers, farmers and scientists. It is open access, free and available online at www.Farmpedia.org
A Picture Book of Best Practices for Subsistence Farmers (2017). By MN Raizada with illustrations by L. Jay Smith (190 pages, 5 versions)
This book consists of 145 best practices (both scientific and indigenous) presented in a graphical format to assist illiterate farmers. It features beautiful illustrations from talented computer graphics artist, L. Jay Smith. The book is open access, copyright-free and available online at SAKbooks.com. Organizations that work with farmers can download compilations of individual lessons to create customized booklets and add their own text translations. Furthermore, 5 ethnic versions of the book have been created, representing the major tropical/subtropical regions of the world.
This book consists of 145 best practices (both scientific and indigenous) presented in a graphical format to assist illiterate farmers. It features beautiful illustrations from talented computer graphics artist, L. Jay Smith. The book is open access, copyright-free and available online at SAKbooks.com. Organizations that work with farmers can download compilations of individual lessons to create customized booklets and add their own text translations. Furthermore, 5 ethnic versions of the book have been created, representing the major tropical/subtropical regions of the world.
Related: A Picture Booklet of Best Practices for Nepalese Farmers (2016).
| sak_picture_booklet_nepali_final_aug8_2016.pdf |
Journal Articles and Book Chapters
These PDFs are meant for personal use only. By downloading one of these PDFs, you are agreeing not to distribute to a third party. Hint: PDFs download much faster if you click on the PDF icon directly.
103. Shrestha, A., Limay-Rios, V., Brettingham, D.J.L. and Raizada M.N. (2023) Maize pollen carry bacteria that suppress a fungal pathogen that enters through the male gamete fertilization route. Frontiers in Plant Science (In revision)
OPEN ACCESS
OPEN ACCESS
102. Khalaf, E.M.*, Shrestha, A.*, Reid, M., McFadyen, B.J. and Raizada M.N. (2023) Conservation and diversity of the pollen microbiome of pan-American maize using PacBio and MiSeq. Frontiers in Microbiology (In press)
(*co-equal first authors)
OPEN ACCESS
(*co-equal first authors)
OPEN ACCESS
101. Shrestha, A., Limay-Rios, V., Brettingham, D. and Raizada MN (2023) Bacteria existing in pre-pollinated styles (silks) can defend the exposed male fertilization channel of maize against an environmental Fusarium pathogen. Frontiers in Plant Science 14, 1292109
https://www.frontiersin.org/articles/10.3389/fpls.2023.1292109/abstract
OPEN ACCESS
https://www.frontiersin.org/articles/10.3389/fpls.2023.1292109/abstract
OPEN ACCESS
100. Thompson, MEH, Shrestha, A., Rinne, J., Limay-Rios, V., Reid, L. and Raizada MN (2023) The cultured microbiome of pollinated maize silks shifts after infection with Fusarium graminearum and varies by distance from the site of pathogen inoculation. Pathogens 12, 1322.
https://www.mdpi.com/2076-0817/12/11/1322
OPEN ACCESS
https://www.mdpi.com/2076-0817/12/11/1322
OPEN ACCESS
99. Pudasaini R., Hewedy O, and Raizada MN (2023) Improving field legume nodulation by crushing nodules onto seeds: implications for small-scale farmers. Frontiers in Agronomy 5, 1161978
https://www.frontiersin.org/articles/10.3389/fagro.2023.1161978/full
OPEN ACCESS
https://www.frontiersin.org/articles/10.3389/fagro.2023.1161978/full
OPEN ACCESS
98. Khadka K, Kaviani M, Raizada MN and Navabi A (2021) Phenotyping and identification of reduced height (Rht) alleles (Rht-B1b and Rht-D1b) in a Nepali spring wheat (Triticum aestivum L.) diversity panel to enable seedling vigor selection. Agronomy 11, 2412
www.mdpi.com/2073-4395/11/12/2412
OPEN ACCESS
www.mdpi.com/2073-4395/11/12/2412
OPEN ACCESS
| 98._khadka_2021_agronomy_rht_coleoptiles.pdf |
97. Dumigan CR, Muileboom J, Gregory J, Shrestha A, Hewedy OA and Raizada MN (2021) Ancient relatives of modern maize from the center of maize domestication and diversification host endophytic bacteria that confer tolerance to nitrogen starvation. Frontiers in Plant Science 12: 660673
OPEN ACCESS
https://www.frontiersin.org/articles/10.3389/fpls.2021.660673/full
OPEN ACCESS
https://www.frontiersin.org/articles/10.3389/fpls.2021.660673/full
| 97._dumigan_frontiers_2021_ancient_nue_microbes.pdf |
96. Thilakarathna MS, Torkamaneh D, Bruce RW, Rajcan I, Chu G, Grainger CM, Szczyglowski, Hill B and Raizada MN (2021) Testing whether pre-pod-fill symbiotic nitrogen fixation in soybean is subject to drift or selection over 100 years of soybean breeding. Frontiers in Agronomy 3: 725813.
OPEN ACCESS
https://www.frontiersin.org/articles/10.3389/fagro.2021.725813/full
OPEN ACCESS
https://www.frontiersin.org/articles/10.3389/fagro.2021.725813/full
| 96._thilakarathna_2021_frontiers_soybean_snf_variation.pdf |
95. Khalaf EM, Shresha A, Rinne J, Lynch MDJ, Shearer CR, Limay-Rios V, Reid LM and Raizada MN (2021) Transmitting silks of maize have a complex and dynamic microbiome. Scientific Reports 11: 13215
https://www.nature.com/articles/s41598-021-92648-4
OPEN ACCESS
https://www.nature.com/articles/s41598-021-92648-4
OPEN ACCESS
| 95._khalaf_et_al-2021-scientific_reports.pdf |
| 95._khalaf_supplementary.pdf |
| 95._khalaf_supplementary_dataset.xlsx |
94. Khadka K, Burt AJ, Earl HJ, Raizada MN and Navabi A (2021) Does leaf waxiness confound the use of NDVI in the assessment of chlorophyll when evaluating genetic diversity panels of wheat? Agronomy 11, 486
www.mdpi.com/2073-4395/11/3/486
OPEN ACCESS
www.mdpi.com/2073-4395/11/3/486
OPEN ACCESS
93. Sousa EC and Raizada MN (2020) Contributions of African crops to American culture and beyond: The Slave Trade and other journeys of resilient peoples and crops. Frontiers in Sustainable Food Systems 4: 586340
https://www.frontiersin.org/articles/10.3389/fsufs.2020.586340/abstract
OPEN ACCESS
https://www.frontiersin.org/articles/10.3389/fsufs.2020.586340/abstract
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92. Devkota R , Hambly Odame H, Fitzsimons J, Pudasaini R, and Raizada MN (2020) Evaluating the Effectiveness of Picture-Based Agricultural Extension Lessons Developed Using Participatory Testing and Editing with Smallholder Women Farmers in Nepal. Sustainability 12, 9699
https://www.mdpi.com/2071-1050/12/22/9699
OPEN ACCESS
https://www.mdpi.com/2071-1050/12/22/9699
OPEN ACCESS
| 92._devkota_2020_sustainability_sak_picture_book.pdf |
91. Khadka K, Torkamaneh D, Kaviani M, Belzile F, Raizada MN and Navabi A (2020)
Population structure of Nepali spring wheat (Triticum aestivum L.) germplasm. BMC Plant Biology 20:530
https://link.springer.com/article/10.1186/s12870-020-02722-8
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Population structure of Nepali spring wheat (Triticum aestivum L.) germplasm. BMC Plant Biology 20:530
https://link.springer.com/article/10.1186/s12870-020-02722-8
OPEN ACCESS
| 91._khadka_2020_bmc_plant_biology_pop_structure_nepali_wheat.pdf |
90. Khalaf EM and Raizada MN. 2020. Draft genome sequences of six strains of Lactococcus lactis (phylum Firmicutes), spanning the seeds of Cucumis sativus L. (cucumber), Cucumis melo L. (cantaloupe), and Cucurbita pepo var.turbinate (acorn squash). Microbiol Resour Announc 9:e00665-20.
https://doi.org/10.1128/MRA.00665-20.
OPEN ACCESS
https://doi.org/10.1128/MRA.00665-20.
OPEN ACCESS
| 90._khalaf_and_raizada_2020_9_37_lactococcus_lactis_from_cucurbit_seeds.pdf |
89. Khalaf EM and Raizada MN. 2020. Draft genome sequences of Pantoea agglomerans, Paenibacillus polymyxa, and Pseudomonas sp.strains, seed biogel-associated endophytes of Cucumis sativus L. (cucumber) and Cucumis
melo L. (cantaloupe). Microbiol Resour Announc 9:e00667-20. https://doi.org/10.1128/MRA.00667-20.
OPEN ACCESS
melo L. (cantaloupe). Microbiol Resour Announc 9:e00667-20. https://doi.org/10.1128/MRA.00667-20.
OPEN ACCESS
| 89._khalaf_and_raizada_2020_9_32_mra_cucumber_and_cantaloupe_biogel.pdf |
88. Khadka K, Raizada MN and Navabi A (2020) Recent Progress in Germplasm Evaluation and Gene Mapping to Enable Breeding of Drought-Tolerant Wheat. Frontiers in Plant Science 11:1149
https://www.frontiersin.org/articles/10.3389/fpls.2020.01149/full
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https://www.frontiersin.org/articles/10.3389/fpls.2020.01149/full
OPEN ACCESS
| 88._khadka_2020_frontiers_wheat_germplasm_evaluation_drought.pdf |
87. Khalaf EM and Raizada MN. 2020. Draft genome sequences of Bacillus and Paenibacillus species isolated from seeds of Citrullus lanata (watermelon), Cucurbita moschata (butternut squash), and Cucurbita pepo L. var. pepo L. (pumpkin). Microbiol Resour Announc 9:e00727-20. https://doi.org/10.1128/MRA.00727-20.
OPEN ACCESS
OPEN ACCESS
| 87._khalaf_and_raizada_2020_9_34_bacillus_and_paenibacillus_from_melon_squash_pumpkin.pdf |
86. Khalaf EM and Raizada MN. 2020. Draft genome sequences of seven strains of Paenibacillus spp. (phylum Firmicutes) inhabiting the seeds of Cucumis melo L. (cantaloupe) and exhibiting plant probiotic traits. Microbiol Resour Announc 9:e00715-20.
https://doi.org/10.1128/MRA.00715-20.
OPEN ACCESS
https://doi.org/10.1128/MRA.00715-20.
OPEN ACCESS
| 86._khalaf_and_raizada_2020_mra_9_34_aenibacillus_from_cantaloupe_seeds.pdf |
85. Khadka K, Earl HJ, Raizada MN and Navabi A (2020) A Physio-Morphological Trait-Based Approach for Breeding Drought Tolerant Wheat. Frontiers in Plant Science 11: 715
https://www.frontiersin.org/articles/10.3389/fpls.2020.00715/full
OPEN ACCESS
https://www.frontiersin.org/articles/10.3389/fpls.2020.00715/full
OPEN ACCESS
| 85._khadka_2020_frontiers_physiol_drought_review.pdf |
84. Devkota R, Pant LP, Gartaula HN, Patel K, Gauchan D, Hambly-Odame H, Thapa B, and Raizada MN (2020) Responsible Agricultural Mechanization Innovation for the Sustainable Development of Nepal’s Hillside Farming System. Sustainability 12, 374 [Selected as Editor's Choice]
https://www.mdpi.com/2071-1050/12/1/374
OPEN ACCESS
https://www.mdpi.com/2071-1050/12/1/374
OPEN ACCESS
| 84._devkota_et_al_2020_small_scale_mechanization_nepal_sustainability.pdf |
83. Chapagain T, Lee EA and Raizada MN (2020) The potential of multi-species mixtures to diversify cover crop benefits. Sustainability 12, 2058.
https://www.mdpi.com/2071-1050/12/5/2058
www.mdpi.com/2071-1050/12/5/2058
OPEN ACCESS
https://www.mdpi.com/2071-1050/12/5/2058
www.mdpi.com/2071-1050/12/5/2058
OPEN ACCESS
| 83._chapagain_2020_cover_crop_multimixes.pdf |
82. Khalaf EM and Raizada MN. 2020. Draft genome sequences of Acinetobacter sp. strain EKM10A, Enterobacter hormaechei EKM10E, and Enterobacter hormaechei EKM11E (phylum Proteobacteria) colonizing the seed surface biogel of Echinocystis lobata (wild cucumber). Microbiol Resour Announc 9:e00184 20.
https://doi.org/10.1128/MRA.00184-20
OPEN ACCESS
https://doi.org/10.1128/MRA.00184-20
OPEN ACCESS
| 82._khalaf_and_raizada_2020_mra_9_20_ekm10a_ekm10e_ekm11e_seed_biogel.pdf |
81. Khalaf EM and Raizada MN. 2020. Draft genome sequence of Bacillus sp. strain EKM601B (phylum Firmicutes), living inside the seeds of Luffa acutangula (Chinese okra). Microbiol Resour Announc 9:e00180-20.
https://doi.org/10.1128/MRA.00180-20.
OPEN ACCESS
https://doi.org/10.1128/MRA.00180-20.
OPEN ACCESS
| 81._khalaf_and_raizada_2020_9_20_mra_ekm601b_luffa_seeds.pdf |
80. Soliman SSM and Raizada MN (2020) Sites of biosynthesis and storage of Taxol in Taxus media (Rehder) plants: Mechanism of accumulation. Phytochemistry 175:112369 (Selected to be on the Cover)
www.sciencedirect.com/science/article/abs/pii/S0031942220300224
www.sciencedirect.com/science/article/abs/pii/S0031942220300224
| 80._soliman_and_raizada_2020_phytochemistry_with_cover_page.pdf |
79. Johnston-Monje, D, Castillo-Avila, DK, Raizada, MN, Becerra Lopez-Lavalle, L.A. (2019) Paying the Rent: How Endophytic Microorganisms Help Plant Hosts Obtain Nutrients. In Comprehensive Biotechnology, Vol. 4, Moo-Young, M., Ed., Elsevier: Pergamon, pp 770–788.
| 79._johnston_monje_2019_endophyte_book_chapter_paying_rent.pdf |
78. Thilakarathna MS and Raizada MN (2019) A biosensor-based assay (GlnLux-agar) shows defoliation triggers rapid release of glutamine from nodules and young roots of forage legumes. Phytobiomes 3:85-91
https://apsjournals.apsnet.org/doi/pdf/10.1094/PBIOMES-03-19-0014-R
OPEN ACCESS
https://apsjournals.apsnet.org/doi/pdf/10.1094/PBIOMES-03-19-0014-R
OPEN ACCESS
| 78._thilakarthna_2019_phytobiomes_glnlux_defoliation.pdf |
77. Chapagain, T., B. Ghimire, R. Pudasaini, K. Gurung, K. Choi, L. Rai, S. Magar, B. BK. and M. N. Raizada (2019) The underutilized terrace wall can be intensified to improve farmer livelihoods. Agronomy for Sustainable Development 39:29
https://link.springer.com/article/10.1007/s13593-019-0574-2
https://link.springer.com/article/10.1007/s13593-019-0574-2
| 77._chapagain2019_article_theunderutilizedterracewallcan.pdf |
76. Thilakarathna MS, Chapagain T, Ghimire B, Pudasaini R, Tamang BB, Gurung K, Choi K, Rai L, Magar S, Bishnu BK, Gaire S, Raizada MN (2019) Evaluating the effectiveness of rhizobium inoculants and micronutrients as technologies for Nepalese common bean smallholder farmers in the real-world context of highly variable hillside environments and indigenous farming practices. Agriculture 9: 20
https://www.mdpi.com/2077-0472/9/1/20/htm
OPEN ACCESS
https://www.mdpi.com/2077-0472/9/1/20/htm
OPEN ACCESS
| 76._thilakarathna_raizada_2019_agriculture_nepal_rhizobia.pdf |
75. Thompson MEH and Raizada MN (2018) Fungal Pathogens of Maize Gaining Free Passage Along the Silk Road. Pathogens 7:81 (Selected as Editor's Choice)
https://www.mdpi.com/2076-0817/7/4/81
OPEN ACCESS
https://www.mdpi.com/2076-0817/7/4/81
OPEN ACCESS
| 75._thompson_and_raizada_2018_silk_pathogens_review.pdf |
74. Dumigan CR, Perry GE, Pauls KP, and Raizada MN (2018) Draft genome sequence of Enterobacter cloacae 3D9 (Phylum Proteobacteria). Microbiology Resource Announcements (In press, DOI: 10.1128/MRA.00902-18)
https://mra.asm.org/content/7/16/e00902-18
OPEN ACCESS
https://mra.asm.org/content/7/16/e00902-18
OPEN ACCESS
| 74._dumigan_raizada_2018_mra_3d9_genome_sequence.pdf |
73. Thilakarathna MS and Raizada MN (2018) Visualizing glutamine accumulation in root systems involved in the legume-rhizobia symbiosis by placement on agar embedded with companion biosensor cells. Phytobiomes (https://doi.org/10.1094/PBIOMES-07-18-0031-TA)
OPEN ACCESS
OPEN ACCESS
Selected as Editor's pick
| 73._thilakarathna_raizada_2018_phytobiomes_glnlux_imaging.pdf |
72. Chapagain T, Ghirmire B, Pudasaini R, Gurung K, Choi K, Rai L, Magar S, Bishnu BK and Raizada MN (2018) Intercropping of maize, millet, mustard, wheat and ginger increased land productivity and potential economic returns for smallholder terrace farmers in Nepal. Field Crops Research 227: 91-101
| 72._chapagain_2018_field_crop_research_nepal_intercropping.pdf |
71. Dumigan CR, Perry GE, Pauls KP, and Raizada MN. 2018. Draft genome sequence of Enterobacter cloacae 3F11 (phylum Proteobacteria). Microbiology Resource Announcements 7:e00846-18.
https://doi.org/10.1128/MRA.00846-18
OPEN ACCESS
https://doi.org/10.1128/MRA.00846-18
OPEN ACCESS
| 71._dumigan_2018_micro_res_announce_3f11_zeanic_p-solubilizing.pdf |
70. Thilakarathna MS and Raizada MN (2018) Challenges in Using Precision Agriculture to Optimize Symbiotic Nitrogen Fixation in Legumes: Progress, Limitations and Future Improvements Needed in Diagnostic Testing. Agronomy 8(5):78
http://www.mdpi.com/2073-4395/8/5/78
OPEN ACCESS
http://www.mdpi.com/2073-4395/8/5/78
OPEN ACCESS
| 70._thilakarathna_2018_agronomy_precision_agriculture_snf.pdf |
69. Soliman SSM and Raizada MN (2018) Darkness: A crucial factor in fungal Taxol production. Frontiers in Microbiology 9: 353
https://www.frontiersin.org/articles/10.3389/fmicb.2018.00353/full
OPEN ACCESS
https://www.frontiersin.org/articles/10.3389/fmicb.2018.00353/full
OPEN ACCESS
| 69._soliman_raizada_2017_frontiers_taxol_light.pdf |
68. Khalaf EM and Raizada MN (2018) Bacterial seed endophytes of domesticated cucurbits antagonize fungal and oomycete pathogens including powdery mildew. Frontiers in Microbiology (Feb 5, 2018)
https://doi.org/10.3389/fmicb.2018.00042
OPEN ACCESS
Viewed 8500 times in the first 2 years since publication.
https://doi.org/10.3389/fmicb.2018.00042
OPEN ACCESS
Viewed 8500 times in the first 2 years since publication.
| 68._khalaf_and_raizada_2018.pdf |
67. Nameth MB, Goron TL, Dinka SJ, Morris A, English J, Lewis D, Oro R, and Raizada MN (2018) The initial hours of post-excision light are critical for adventitious root regeneration from Arabidopsis thaliana (L.) Heynh. cotyledon explants. In Vitro Cellular & Developmental Biology - Plant 54(3): 273-290
https://link.springer.com/article/10.1007/s11627-017-9880-z
https://link.springer.com/article/10.1007/s11627-017-9880-z
| 67._nameth_2018_in_vitro_light_root_regeneration.pdf |
Selected as a Journal Highlight in the In Vitro Report, an official publication of the Society for In Vitro Biology
66. Raizada MN (2017) Challenges and Solutions for Subsistence Farmers. In Plants, Genes and Agriculture: Sustainability through Biotechnology (Maarten J. Chrispeels and Paul Gepts, Editors). Sinauer Assoc. Inc. and Oxford University Press.
New undergraduate university textbook
Amazon link
New undergraduate university textbook
Amazon link
65. Shehata HR, Dumigan C, Watts S and Raizada MN (2017) An endophytic microbe from an unusual volcanic swamp corn seeks and inhabits root hair cells to extract rock phosphate. Scientific Reports 7:13479
OPEN ACCESS
OPEN ACCESS
| 65._shehata_et_al-2017-scientific_reports_published_pdf_copy.pdf |
Selected as one of the top25 most accessed articles in Plant Science in this journal in 2017 (within the Top 100 List).
64. Shehata HR, Lyons EM and Raizada MN (2017) Turfgrasses as model assay systems for high-throughput in planta screening of beneficial endophytes isolated from cereal crops. Symbiosis (DOI: 10.1007/s13199-017-0511-6)
| 64._shehata_2017_symbiosis_turf_model_system.pdf |
63. Thilakarathna MS, Moroz N and Raizada MN (2017) A biosensor-based leaf punch assay for glutamine correlates to symbiotic nitrogen fixation measurements in legumes to permit rapid screening of rhizobia inoculants under controlled conditions. Frontiers in Plant Science 8: 1714
OPEN ACCESS
This study uses our microbial biosensor-based GlnLux precision agriculture invention.
OPEN ACCESS
This study uses our microbial biosensor-based GlnLux precision agriculture invention.
| 63._thilakarathna_and_raizada_2017_frontiers_glnlux_leaf_assay.pdf |
62. Goron TL and Raizada MN (2017) Biosensor-mediated in situ imaging defines the availability period of assimilatory glutamine in maize seedling leaves following nitrogen fertilization. Nitrogen 1:2
OPEN ACCESS
This study uses our microbial biosensor-based GlnLux precision agriculture invention.
OPEN ACCESS
This study uses our microbial biosensor-based GlnLux precision agriculture invention.
| 62._goron_et_al_2017_nitrogen_pulse_chase_.pdf |
61. Shehata HR and Raizada MN (2017) A Burkholderia endophyte of the ancient maize landrace Chapalote utilizes c-di-GMP-dependent and –independent signaling to suppress diverse plant fungal pathogen targets. FEMS Microbiology Letters 364: fnx138
60. Goron TL, Nederend J, Stewart G, Deen B, and Raizada MN (2017) Mid-season leaf glutamine predicts end-season maize grain yield and nitrogen content in response to nitrogen fertilization under field conditions. Agronomy 7(2):41
OPEN ACCESS
This study uses our microbial biosensor-based GlnLux precision agriculture invention.
OPEN ACCESS
This study uses our microbial biosensor-based GlnLux precision agriculture invention.
| 60._goron_raizada_agronomy_glnlux_field.pdf |
59. Chapagain T and Raizada MN (2017) Impacts of natural disasters on smallholder farmers: Gaps and recommendations. Agriculture and Food Security 6:39
OPEN ACCESS
This paper introduces the concept of low cost, emergency Sustainable Agriculture Kits (eSAKs) to help farmers after a disaster.
Featured in a news release by the University of Guelph:
https://news.uoguelph.ca/2017/05/profs-innovation-aids-natural-disaster-recovery/
Viewed >20,000 times in the first 3 years since publication.
OPEN ACCESS
This paper introduces the concept of low cost, emergency Sustainable Agriculture Kits (eSAKs) to help farmers after a disaster.
Featured in a news release by the University of Guelph:
https://news.uoguelph.ca/2017/05/profs-innovation-aids-natural-disaster-recovery/
Viewed >20,000 times in the first 3 years since publication.
| 59._chapagain_raizada_2017_agric_food_security_esak_disaster.pdf |
58. Chapagain T and Raizada MN (2017) Agronomic challenges and opportunities for smallholder terrace agriculture in developing countries. Frontiers in Plant Science 8: 331 (provisional PDF online. doi: 10.3389/fpls.2017.00331)
OPEN ACCESS
Viewed nearly 22,000 times in the first 3 years since publication.
OPEN ACCESS
Viewed nearly 22,000 times in the first 3 years since publication.
| 58._chapagain_and_raizada_2017_frontiers_in_plant_science_terrace_farming_review.pdf |
| small_et_al-2017-agriculture___food_security.pdf |
56. Raizada MN, Goron TL, Bannerjee O, Quach Mason M, Pautler M, Brazolot J, Morris A, Kajenthira A, Dinka S, DiMeo N (2017) Loss of developmental pluripotency occurs in two stages during leaf aging in Arabidopsis thaliana. In Vitro Cellular & Developmental Biology - Plant 53: 178-187
| 56._raizada_2017_in_vitro_age_pluripotency.pdf |
55. Shehata HR, Griffiths MW and Raizada MN (2017) Seeds of the wild progenitor of maize possess bacteria that antagonize foodborne pathogens. Foodborne Pathogens and Disease 14: 4 (ePub Feb 10, 2017 doi:10.1089/fpd.2016.2225)
| 55._shehata_food_pathogens_disease_2017.pdf |
| 54._thilakarathna_and_raizada_2017_-_soybean_inoculant_review.pdf |
53. Quach Mason, M, Goron TL, Dziewiecka Arnold E, Dickinson P, Kajenthira A, Dinka SJ, Oro R, and Raizada MN (2017) Pyramid screening: combining three genetic screens into one efficient screen for shoot regeneration mutants in Arabidopsis thaliana. Journal of Plant Growth Regulation (Online Jan 21, 2017 doi:10.1007/s00344-016-9651-9)
| 53._quach_2017_j_plant_growth_regulation_pyramid_screening.pdf |
52. Goron TL and Raizada MN (2016) Biosensor-based spatial and developmental mapping of maize leaf glutamine at vein-level resolution in response to different nitrogen rates and uptake/assimilation durations. BMC Plant Biology 16:230
OPEN ACCESS
This study uses our microbial biosensor-based GlnLux precision agriculture invention.
OPEN ACCESS
This study uses our microbial biosensor-based GlnLux precision agriculture invention.
| 53._goron_raizada_2016_bmc_plant_biology_glnlux_maize_vein_resolution.pdf |
| 52._thilakarathna_2016_agron_sustain_dev_n_transfer.pdf |
| shehata_2016_frontiers_in_microbiology_chapalote_endophyte_1000bc.pdf |
49. Mousa WK, Shearer C, Limay-Rios V, Ettinger CL, Eisen JA, Raizada MN (2016) Root hair-endophyte stacking (RHESt) in finger millet creates a physico-chemical barrier to trap the fungal pathogen Fusarium graminearum. Nature Microbiology 1: 16167 (Article)
A preprint of this paper is available for free at http://biorxiv.org/content/early/2016/08/25/071548
Cool finger millet root video taken by Walaa Mousa of the phenomenon: The root is the vertical purple-red column on the right. Here Fusarium fungal pathogen was added at a distance at the upper left (not visible), and in response, seed-coated endophytic bacteria M6 (GFP-labelled, green) has swarmed towards it, apparently exited, and multiplied dramatically on the left surface of the root (rhizoplane). In parallel, M6 has apparently induced dramatic growth of root hairs (long purple strands on the left, bending vertically and forming a thick mat) onto which the endophyte cells are starting to stack as green layers, similar to a lasagna (in the analogy, the root hairs are the red noodle sheets, and the filling is the endophytic bacteria in green). As a result, multiple barriers are formed against the Fusarium pathogen. Within this complex, M6 produces potent fungicides to kill invading Fusarium so that it cannot enter into the root.
The significance of this discovery is explained in our Press Coverage:
Press coverage - Radio interview - Canadian Broadcasting Corporation (CBC) "Quirks and Quarks"
Press coverage - UofG Press Release
Press coverage - Science Daily
Press coverage - Nature Middle East
A preprint of this paper is available for free at http://biorxiv.org/content/early/2016/08/25/071548
Cool finger millet root video taken by Walaa Mousa of the phenomenon: The root is the vertical purple-red column on the right. Here Fusarium fungal pathogen was added at a distance at the upper left (not visible), and in response, seed-coated endophytic bacteria M6 (GFP-labelled, green) has swarmed towards it, apparently exited, and multiplied dramatically on the left surface of the root (rhizoplane). In parallel, M6 has apparently induced dramatic growth of root hairs (long purple strands on the left, bending vertically and forming a thick mat) onto which the endophyte cells are starting to stack as green layers, similar to a lasagna (in the analogy, the root hairs are the red noodle sheets, and the filling is the endophytic bacteria in green). As a result, multiple barriers are formed against the Fusarium pathogen. Within this complex, M6 produces potent fungicides to kill invading Fusarium so that it cannot enter into the root.
The significance of this discovery is explained in our Press Coverage:
Press coverage - Radio interview - Canadian Broadcasting Corporation (CBC) "Quirks and Quarks"
Press coverage - UofG Press Release
Press coverage - Science Daily
Press coverage - Nature Middle East
| 49._mousa_raizada_2016_nature_microbiology_small.pdf |
| 49._mousa_raizada_2016_supplementary_info_nature_microbiology_small.pdf |
| 48._mousa_2016_molecules_antifungal_natural_products.pdf |
47. Khalaf EM and Raizada MN (2016) Taxonomic and functional diversity of cultured seed associated microbes of the cucurbit family. BMC Microbiology 16: 131
OPEN ACCESS
OPEN ACCESS
| 47._khalaf_and_raizada_2016_bmc_microbiology_cucurbit_seed_endophytes.pdf |
| 46._jonston_monje_2016_plant_soil_soil_and_seed_originating_endophytes_maize.pdf |
| 45._mousa_2016_grain_disease_book_chapter.pdf |
| shehata_2016_jam_anti_dollar_spot_endophytes.pdf |
| shehata_2016_bmc_microbiology_in_vitro_vs_in_planta_screens.pdf |
| 43._mousa_2015_frontiers_maize_fungal_endophyte_wf4_against_fusarium.pdf |
| 42._mousa_et_al_frontiers_in_plant_sci__2015__maize_endophytes_against_fusarium.pdf |
40. Soliman SM, Greenwood JS, Bombarely A, Mueller LA, Tsao R, Mosser DD and Raizada MN (2015) An endophyte constructs fungicide-containing extracellular barriers for its host plant. Current Biology 25, 1-7
OPEN ACCESS
-This paper, later selected as the Editor's Choice Discovery in Plant Biology by The Scientist magazine, describes a novel symbiotic relationship between a plant and a fungus in which the fungus creates a molecular bandage laced with a fungicide to seal and protect wounds. The fungicide is the important anti-cancer drug Taxol. More generally, the paper also proposes that plants have selected for mobile microbes to inhabit their vascular tissues in order to search, swarm and kill pathogens, since plant cells themselves cannot move -- a type of convergent evolution analogous to mammalian immunity cells.
Article Impact
Press Release
Article coverage on ScienceDaily
OPEN ACCESS
-This paper, later selected as the Editor's Choice Discovery in Plant Biology by The Scientist magazine, describes a novel symbiotic relationship between a plant and a fungus in which the fungus creates a molecular bandage laced with a fungicide to seal and protect wounds. The fungicide is the important anti-cancer drug Taxol. More generally, the paper also proposes that plants have selected for mobile microbes to inhabit their vascular tissues in order to search, swarm and kill pathogens, since plant cells themselves cannot move -- a type of convergent evolution analogous to mammalian immunity cells.
Article Impact
Press Release
Article coverage on ScienceDaily
| 41._soliman_2015_current_biology.pdf |
| talbot_n_2015_current_biology_soliman_highlight_paper.pdf |
Soliman et al (2015) article selected as the Hot Topic by the American Society of Pharmacognosy (The ASP Newsletter, volume 52, issue 1, year 2016) see page 13
| 40c._hot_topics_in_pharmacogosy_2016_soliman_p13.pdf |
| 40._harding_and_raizada_2015_frontiers_anti-weed_microbes_review.pdf |
| 39._goron_raizada_2015_frontiers_finger_millet_n_responses.pdf |
| 38._thilakarathna_and_raizada_2015_finger_millet_nutrient_review.pdf |
36. Goron TL, Watts S, Shearer C and Raizada MN (2015) Growth in Turface clay permits root hair phenotyping along the entire crown root zone in cereal crops and demonstrates that root hair growth can extend well beyond the root hair zone. BMC Research Notes 8:143
OPEN ACCESS
| 37._goron_2015_root_hair_bmc_research_notes.pdf |
35. Mousa WK and Raizada MN (2015) Biodiversity of genes encoding anti-microbial traits within plant associated microbes. Frontiers in Plant Science 6: 231
OPEN ACCESS
| 36._mousa_raizada_2015_frontiers_microbe_gene_diversity_review.pdf |
| 35._goron_raizada_2015_frontiers_millet_review.pdf |
| 34._ettinger_mousa_2015_genome_announcements_finger_millet_m6.pdf |
| 33._ettinger_shehata_2015_genome_announcements_a12.pdf |
| 32._johnston_monje_et_al_2014_bmc_plant_biology_soil_swap.pdf |
30. Goron, T.L. and Raizada, M.N. (2014) Current and Future Transgenic Whole-Cell Biosensors for Plant Macro- and Micro-nutrients. Critical Reviews in Plant Sciences 33: 392-413
-This comprehensive review describes novel tools to help farmers and researchers measure bioavailable soil nutrients including nitrogen
-This comprehensive review describes novel tools to help farmers and researchers measure bioavailable soil nutrients including nitrogen
| 30._goron_raizada_2014_critical_reviews_plant_sciences_nutrient_biosensor_review.pdf |
| 29._gaudin_raizada_2014_bmc_genetics_tb1.pdf |
28. Liseron-Monfils C., Bi Y.-M., Downs G.S., Wu W., Signorelli T., Lu G., Chen X., Bondo E., Zhu T., Lukens L.N., Colasanti J., Rothstein, S.J. and Raizada M.N. (2013) Nitrogen transporter and assimilation genes exhibit developmental stage-selective expression in maize (Zea mays L.) associated with distinct cis-acting promoter motifs. Plant Signaling & Behavior 8: e26056
OPEN ACCESS
| 28._liseron_monfils_raizada_2013_plant_signal_behavior_maize_n_transcriptome.pdf |
27. Bargout, R.N. and Raizada, M.N. (2013) Soil Nutrient Management in Haiti, Pre-Columbus to the Present Day: Lessons for Future Agricultural Interventions. Agriculture & Food Security 2:11
OPEN ACCESS
-This comprehensive review on Haiti includes recommendations for inexpensive approaches to reduce synthetic nitrogen fertilizer requirements in Haiti, such as for maize (corn) farmers, including the use of rhizobia inoculants for intercropped legumes.
-The number of article views is approaching 25,000 since publication, and the paper is currently ranked in the Top10 most highly viewed articles in this journal since its inception in 2012.
-The number of article views is approaching 25,000 since publication, and the paper is currently ranked in the Top10 most highly viewed articles in this journal since its inception in 2012.
| 27._bargout_and_raizada_2012_agric_food_security_haiti.pdf |
26. Soliman, S.S.M., Trobacher, C.P., Tsao, R., Greenwood, J. and Raizada, M.N. (2013) A Fungal Endophyte Induces Transcription of Genes Encoding a Redundant Fungicide Pathway in its Host Plant. BMC Plant Biology 13:93
OPEN ACCESS
| 26._soliman_raizada_2013_bmc_plant_biology_taxus_endophyte_plant_interact.pdf |
25. Berchie, J.N., Gaudin, A., McClymont, S. Raizada, M. Adu-Dapaah, H. and Sarkodie-Addo, J. (2013) Performance of 13 Bambara Groundnut (Vigna subterranea (L) Verdc.) Landraces (from Sub-Saharan Africa) under 12 h and 14 h Photoperiod. Journal of Agronomy 12: 20-28
OPEN ACCESS
-A collaboration with the Kwame Nkrumah University of Science and Technology in Ghana (West Africa), funded by the Canadian Department of Foreign Affairs. Bambara is one of Africa's most ancient, nutritious, drought tolerant legume crops, but neglected. It effectively associates with nitrogen fixing microbes and deposits large amounts of organic nitrogen into soil and holds significant potential to combat soil degradation, climate change and malnutrition.
OPEN ACCESS
-A collaboration with the Kwame Nkrumah University of Science and Technology in Ghana (West Africa), funded by the Canadian Department of Foreign Affairs. Bambara is one of Africa's most ancient, nutritious, drought tolerant legume crops, but neglected. It effectively associates with nitrogen fixing microbes and deposits large amounts of organic nitrogen into soil and holds significant potential to combat soil degradation, climate change and malnutrition.
| 25._berchie_et_al_2013_j_agronomy_bambara_photoperiod.pdf |
24. Mousa, W.K. and Raizada, M.N. (2013) The diversity of anti-microbial secondary metabolites produced by fungal endophytes: An interdisciplinary perspective. Frontiers in Microbiology 4:65
OPEN ACCESS
-This review focuses on anti-pathogenic natural chemicals produced by beneficial microbes that inhabit plants.
-The article has been viewed 20,000 times since publication
-The article has been viewed 20,000 times since publication
| 24._mousa_raizada_2013_frontiers_endophyte_anti-microbe_review.pdf |
23. Liseron-Monfils, C., Lewis, T., Ashlock, D., McNicholas, P.D., Fauteaux, F., Stromvik, M. and Raizada, M.N. (2013) Promzea: A pipeline for discovery of regulatory motifs in maize and other plant species and its application to the anthocyanin and phlobaphene biosynthetic pathways and the Maize Development Atlas. BMC Plant Biology 13: 42
OPEN ACCESS
-This paper describes a new genomics software program called Promzea that is free and available online for the research community.
| 23._liseron_monfils_2013_bmc_plant_biology_promzea_software.pdf |
22. Soliman, S.S.M. and Raizada, M.N. (2013) Interactions between co-habitating fungi elicit synthesis of Taxol from an endophytic fungus in host Taxus plants. Frontiers in Microbiology 4(3): 1-14
OPEN ACCESS
-Taxol (Paclitaxel) is a natural fungicide as well as one of the world's most important anti-cancer drugs, but expensive, especially in developing nations.
| 22._soliman_raizada_2013_frontiers_microbiology_taxol.pdf |
21. Nameth, M.B., Dinka, SJ, Chatfield, SP, Morris, A, English, J, Lewis, D., Oro, R. and Raizada, M.N. (2013) The shoot regeneration capacity of excised Arabidopsis cotyledons is established during the initial hours after injury and is modulated by a complex genetic network of light signalling. Plant Cell & Environment 36: 68-86.
OPEN ACCESS
In vitro plant regeneration from stem cells is important both for commercial plant biotech as well as for "low tech" efforts in developing nations to remove viruses and other pathogens by simply heating plant tissue culture planting materials, thus reducing the need for pesticides. Unfortunately, plant regeneration is very plant cultivar specific and there is considerable variability in regeneration rates. This paper explains part of the underlying problem and suggests simple, low cost strategies to move forward.
| 21._nameth_2013_plant_cell_environment_arabidopsis_light.pdf |
| 20._chatfield_2013_stem_cell_regeneration_plant_j.pdf |
19. Johnston-Monje D. and Raizada M.N. (2013) A survey of diverse Zea seeds for bacterial endophytes. In: F.J. de Bruijn (ed.), Molecular Microbial Ecology of the Rhizosphere. Wiley-Blackwell. Chapter 42, p445-455.
| 19._johnston_monje_raizada_2013_book_chapter_proofs.pdf.zip |
18. Tessaro, M.J., Soliman, S.S.M. and Raizada, M.N. (2012) A whole cell biosensor for glutamine and applications for quantifying and visualizing glutamine in plants. Applied and Environmental Microbiology 78: 604-606.
OPEN ACCESS
This paper describes a novel nitrogen technology invented in our lab which has a number of promising applications including as a sensitive test for soil nitrogen fertilizer availability and as a potential tool for breeders to reduce the cost of breeding for more nitrogen fertilizer efficient crops.
| 18._tessaro_2012_appl._environ._microbiol._glnlux.pdf |
17. Taheri, A., Subramanian, J., Cline, J.A., Raizada, M.N. and Pauls, K.P. (2012) A WD-repeat gene from peach (Prunus persica L.) is a functional ortholog of Arabidopsis thaliana TRANSPARENT TESTA GLABRA1. In Vitro Cellular and Developmental Biology – Plant. 48:23-29.
| 17._taheri_et_al._in_vitro_2012.pdf |
16. Soliman, S.S.M., Tsao, R. and Raizada, M.N. (2011) Chemical inhibitors suggest endophytic fungal paclitaxel is derived from both mevalonate and non-mevalonate-like pathways. Journal of Natural Products 74: 2497–2504.
-First evidence that non-mevalonate enzymes exist in fungi, previously thought to only exist in bacteria and chloroplasts (plants)
-Paclitaxel (Taxol) is a natural fungicide as well as one of the world's most important anti-cancer drugs, but expensive, especially in developing nations.
-Paclitaxel (Taxol) is a natural fungicide as well as one of the world's most important anti-cancer drugs, but expensive, especially in developing nations.
| 16._soliman_2011_jnp_final.pdf |
15. Gaudin, A.C.M., S.A. McClymont and Raizada M.N. (2011) The nitrogen adaptation strategy of the wild teosinte ancestor of modern maize, Zea mays subsp. parviglumis. Crop Science 51: 2780-2795
-Article highlighted by the Crop Science Society of America, Agronomy Society of America and Soil Science Society of America
-2012 Recommended "Hot Papers" by the Editorial Board of the Maize Genetics and Genomics Database (Maize GDB)
-Article highlighted by the Crop Science Society of America, Agronomy Society of America and Soil Science Society of America
-2012 Recommended "Hot Papers" by the Editorial Board of the Maize Genetics and Genomics Database (Maize GDB)
| gaudin_2011_crop_science_teosinte_n_response.pdf |
| 15a._gaudin_2011_crop_science_suppl_1.pdf |
| gaudin_2011_pce_n_maize_response.pdf |
13. Johnston-Monje D. and Raizada M.N. (2011) Plant and Endophyte Relationships: Nutrient Management. In: Murray Moo-Young (ed.), Comprehensive Biotechnology, Second Edition, volume 4, pp. 713–727. Elsevier.
-This paper critically reviews the literature concerning endophytic microbes as inoculants to reduce fertilizer costs.
| 13._johnston_monje_raizada_2011_endophyte_review.pdf |
12. Johnston-Monje D. and Raizada M.N. (2011) Conservation and diversity of seed associated endophytes in Zea across boundaries of evolution, ethnography and ecology. PLoS One 6: e20396.
OPEN ACCESS
-Cited 366x as of 2020
-This paper describes the natural microbes that inhabit ancient and modern corn and which hold potential as low cost, sustainable technologies to reduce fertilizer and pesticide costs of crop production.
| 12._johnston_monje_and_raizada_2011_plos_one_copy.pdf |
11. Chatfield, S.P and Raizada, M.N. (2008) Ethylene and shoot regeneration: hookless1 modulates de novo shoot organogenesis in Arabidopsis thaliana. Plant Cell Reports 27: 655-666
| 11._chatfield_2008_ethylene_regeneration_plant_cell_rep.pdf |
10. Dinka, S.J., Campbell, M.A., Demers, T. Raizada, M.N. (2007) Predicting the Size of the Progeny Mapping Population Required to Positionally Clone a Gene. Genetics 176: 2035-2054.
OPEN ACCESS
| 10._dinka_2007_positional_cloning_rice_genetics.pdf |
9. Singh PM, Wight CA, Sercinoglu O, Wilson DC, Boytsov A, Raizada M.N. (2007) Language Preferences on Websites and in Google Searches for Human Health and Food Information. Journal of Medical Internet Research 9(2):e18
OPEN ACCESS
-A collaboration with Google Labs to measure and improve accessibility of agriculture and health information globally including in developing nations.
| 9._singh_2007_language_internet_jmir.pdf |
8. Dinka, S.J., and Raizada, M.N. (2006) Inexpensive fine mapping and positional cloning in plants using visible, mapped transgenes. Canadian Journal of Botany 84:179-188.
OPEN ACCESS
| 8._dinka_2006_tmars_can_j_bot.pdf |
7. M.N. Raizada (2003) RescueMu Protocols for Maize Functional Genomics. In Methods in Molecular Biology Vol 236: Plant Functional Genomics, Chapter 3, pp.37-58 (E. Grotewold, ed.), Humana Press, New Jersey.
| 7._raizada_2003_rescuemu_plant_functional_genomics.pdf |
PhD Papers
6. M.N. Raizada, G.-L. Nan and V. Walbot (2001) Somatic and Germinal Mobility of the RescueMu Transposon in Transgenic Maize. Plant Cell 13, 1587-1608.
OPEN ACCESS
•Nominated for the Best Paper of the Year Award by a Young Scientist, American Society of Plant Biologists (ASPB)
| 6._raizada_2001_rescuemu_plant_cell.pdf |
5. M.N. Raizada, M.-I. Benito and V. Walbot (2001) The MuDR Transposon Terminal Inverted Repeat Contains a Complex Plant Promoter Directing Distinct Somatic and Germinal Programs. Plant Journal 25, 79-91.
| 5._raizada_2001_mutator_plantj.pdf |
| 4._raizada_2001_mutator_mol_genet_genomics.pdf |
| 3._raizada_2000_35s_mudra_plant_cell.pdf |
Undergraduate Papers
2. R.A. Bouchard, J.R.H. Frappier, L. Liu, M. Raizada, B.G. Atkinson, and D.B. Walden (1993) Developmentally-modulated Expression of Transcripts from Stress-Inducible Gene Families During Microsporogenesis and Gametophyte Development in Zea mays L. Maydica 38, 135-144
| 1._atkinson_raizada_1993_dev_genetics_heat_shock_proteins.pdf |
Software Packages Released
Liseron-Monfils C. and Raizada M.N. (2012) Promzea: A Perl-based application to discover de novo motifs underlying co-regulated genes in maize (www.promzea.org and http://131.104.92.180/).
Liseron-Monfils C. and Raizada M.N. (2012) Promzea: A Perl-based application to discover de novo motifs underlying co-regulated genes in maize (www.promzea.org and http://131.104.92.180/).