Equipment and Facilities Available to Raizada Lab Personnel
The Raizada Lab is fortunate to have access to extensive, new equipment and facilities, either within the lab or walking distance, including $2 million in common-use equipment and a $1.3
million 8-house transgenic greenhouse facility co-designed by Manish for transgenic maize (to add to 8 existing greenhouses and 10 indoor growth rooms). In addition to $300,000 of equipment recently purchased by the Raizada Lab, all personnel have access to 3 common equipment rooms (on each floor). Special thanks to Prof. Peter Pauls who has tirelessly spearheaded equipment- and facility-building efforts on behalf of the Department with generous funding from the Canadian Foundation for Innovation (CFI) and Ontario Innovation Trust (OIT).
The Raizada Lab is fortunate to have access to extensive, new equipment and facilities, either within the lab or walking distance, including $2 million in common-use equipment and a $1.3
million 8-house transgenic greenhouse facility co-designed by Manish for transgenic maize (to add to 8 existing greenhouses and 10 indoor growth rooms). In addition to $300,000 of equipment recently purchased by the Raizada Lab, all personnel have access to 3 common equipment rooms (on each floor). Special thanks to Prof. Peter Pauls who has tirelessly spearheaded equipment- and facility-building efforts on behalf of the Department with generous funding from the Canadian Foundation for Innovation (CFI) and Ontario Innovation Trust (OIT).
Equipment of Interest & User Manuals
1. Roper ChemiProHT Luminescence Imaging System
(designed especially for plants by JKZhu lab; cryo-cooled for long exposures, elimination of dark noise)
(designed especially for plants by JKZhu lab; cryo-cooled for long exposures, elimination of dark noise)
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2. Berthold MicoLumatPlus LB96V 96well Luminometer
(ultra-sensitive; 3 injectors for flash assays)
(ultra-sensitive; 3 injectors for flash assays)
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3. BioTek Flx800TBI top down fluorescence plate reader
(filter wheel-based; lab has 4 excitation/emission filter sets installed)
(filter wheel-based; lab has 4 excitation/emission filter sets installed)
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4. Lightools Illuminatool LT9900 Epifluorescent System + Excitation + Filter sets for GFP/YFP/CFP/RFP
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5. Genesys 20 Visible Spectrophotometer
Range: 325nm to 1100 nm
(there is a UV spec in the 3rdfloor common room)
Range: 325nm to 1100 nm
(there is a UV spec in the 3rdfloor common room)
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6. MJ Research PTC200 Gradient PCR and Dual Alpha Unit Machines
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7. Hoefer DynaQuant DNA Fluorometer for Quantifying dsDNA
(with Hoescht 33258 dye; only sensitive for plasmid prep or genomic DNA isolations >50 ng/uL; otherwise use RiboGreen dye with BioTek Flx800)
(with Hoescht 33258 dye; only sensitive for plasmid prep or genomic DNA isolations >50 ng/uL; otherwise use RiboGreen dye with BioTek Flx800)
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8. BioRad PDS 1000/He plant transformation gun
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9. BioRad Mini Protean 3 gel and transblot system
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10. BioRad Mini Protean 3 Western transblot system
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11. BioRad Mini Protean 2D gel system
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12. BioRad MiniPulser Electroporation System
(for bacteria, yeast, not plant cells)
(for bacteria, yeast, not plant cells)
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13. Barnstead E-pure water purification system
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14. Denver Instruments UltraBasic Benchtop pH metre
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