Raizada Lab Protocols (sorry...needs updating)
Introductory Tutorials and Links to Online Resources:
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A. Arabidopsis resources and molecular techniques I | |
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C. Molecular techniques II and resources | |
File Size: | 1583 kb |
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D. Plant genomics tutorial and resources | |
File Size: | 140 kb |
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G. Molecular, other software tools and online links | |
File Size: | 57 kb |
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Lab Protocols:
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1. Luciferase imaging – Versarray 1300B Specs | |
File Size: | 75 kb |
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2. Luciferase imaging – Science STKE Protocol from JKZhu’s Lab | |
File Size: | 1586 kb |
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3. Luciferase imaging – review Plant Mol Biol Rep (2000) 18, 143a-t | |
File Size: | 2806 kb |
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4. Luciferase imaging – gene trapping, Arabidopsis, Plant J (2003) 35, 273-283 | |
File Size: | 344 kb |
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5. Luciferase imaging – simple explanation from Kevin Smith, undergrad thesis | |
File Size: | 273 kb |
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6. Luciferase imaging – excellent chapter Versarray system in Lum Biotech book | |
File Size: | 159 kb |
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7. Luciferase plant tissue extraction, single cell luminometer protocol – M.Raizada | |
File Size: | 46 kb |
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8. Luciferase -extraction and quantification – K.Luehrsen and V.Walbot, Stanford | |
File Size: | 69 kb |
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9. Luciferase – use of Berthold MicroLumatPlus 96 well luminometer – M.Raizada | |
File Size: | 59 kb |
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10. Flx800TBI Fluorescence Reader – Quick Protocol – M.Raizada | |
File Size: | 58 kb |
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11. Flx800TBI Fluorescence Reader – Chemiluminescence detection theory | |
File Size: | 184 kb |
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12. Flx800TBI Fluorescence Reader - dsDNA quantification using picoGreen | |
File Size: | 126 kb |
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13. Flx800TBI Fluorescence Reader – ssDNA quantification using oliGreen | |
File Size: | 126 kb |
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14. Flx800TBI Fluorescence Reader – RNA quantification using riboGreen | |
File Size: | 137 kb |
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15. Flx800TBI Fluorescence Reader – protein quantification overview | |
File Size: | 111 kb |
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16. Flx800TBI Fluorescence Reader – protein quantification using OPA | |
File Size: | 101 kb |
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17. Flx800TBI Fluorescence Reader – protein quantification using CBCBQ | |
File Size: | 76 kb |
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18. Flx800TBI Fluorescence Reader – quantifying calcium | |
File Size: | 197 kb |
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19. Flx800TBI Fluorescence Reader – GFP quantification notes | |
File Size: | 173 kb |
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20. Flx800TBI Fluorescence Reader – GFP quantification protocol | |
File Size: | 263 kb |
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21. Hoefer Fluorometer/Flx800TBI – dsDNA quantification using Hoescht 33258 | |
File Size: | 259 kb |
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22. Hoefer Fluorometer/Flx800TBI – enhanced dsDNA quantification Hoescht | |
File Size: | 103 kb |
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23. Hoefer Fluorometer – GUS quantification, Stockinger Lab, Ohio State | |
File Size: | 55 kb |
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24. Protein quantification, Bradford Assay,SpectraMax Plate Reader, M.Raizada | |
File Size: | 36 kb |
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25a. Maize - RescueMu protocols, Plant Functional Genomics 2003, M.Raizada | |
File Size: | 792 kb |
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26. Maize – biolistic bombardment, M.Raizada ( | |
File Size: | 51 kb |
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27. Agarose gels, Stockinger Lab, Ohio State | |
File Size: | 29 kb |
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28. E.coli – heat shock transformation (M.Raizada) | |
File Size: | 37 kb |
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29. E.coli – glycerol freezer stocks, Stockinger Lab, Ohio State | |
File Size: | 75 kb |
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30. E.coli – directed protein evolution protocols, F.H. Arnold Lab, Caltech | |
File Size: | 45 kb |
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36b. Arabidopsis - General growth and maintenance M.Raizada (PDF36) | |
File Size: | 50 kb |
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37. Arabidopsis – seed sterilization, Steve Chatfield, Raizada Lab | |
File Size: | 35 kb |
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38. Arabidopsis regeneration – cotyledon regeneration protocol M.Raizada | |
File Size: | 46 kb |
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39. Arabidopsis regeneration – germination and regeneration media M.Raizada | |
File Size: | 44 kb |
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40. Arabidopsis regeneration – regeneration score sheet, Raizada Lab | |
File Size: | 78 kb |
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41. Arabidopsis regeneration – tissue-scanning protocol, Raizada Lab | |
File Size: | 49 kb |
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42. Arabidopsis – Agrobacterium transformation, Stockinger Lab, Ohio State | |
File Size: | 71 kb |
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43. Arabidopsis – Agrobacterium transformation, Andrew Bent, Plant J 1998 | |
File Size: | 223 kb |
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44. Arabidopsis – Agrobacterium competent cells/protocols, TAIR website | |
File Size: | 37 kb |
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45. Arabidopsis – Agrobacterium high-throughput transformation S.Cutler | |
File Size: | 10 kb |
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46._arab_bialophos_protocols.pdf | |
File Size: | 154 kb |
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47. Arabidopsis – inexpensive DNA isolation mapping Plant Methods 2005 | |
File Size: | 838 kb |
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48. Arabidopsis – ploidy analysis protocol, David Galbraith, Plant Methods 2005 | |
File Size: | 749 kb |
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49. Raizada Lab task checklist | |
File Size: | 34 kb |
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50. E.coli - use of ssDNA/tRNA to increase transformation efficiency | |
File Size: | 38 kb |
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51. Steve Chatfield’s Guide to Working with Arabidopsis | |
File Size: | 550 kb |
File Type: | doc |